Dry Powder Cell Culture Medium Processing
In response to the growing demand for dry powder cell culture medium production, Purdue has launched a self-developed cell culture medium process system, which combines needle crusher and cone mixer to meet customers' various dry powder material crushing needs.
Keyword： Dry Powder Cell Culture Medium Processing
The working principle of the hammer mill is that the material enters the crushing bin, and the powder is impacted by the high-speed rotating grinding knife, mainly by the impact effect to crush the material. The material enters the hammer mill and is crushed by the impact of the high-speed rotating hammer head. The crushed material obtains kinetic energy from the hammer head of the hammer mill and rushes to the baffle and screen bar in the frame body from the high-speed. At the same time, the materials collide with each other and are crushed many times. The materials smaller than the gap of the screen bar are discharged from the gap. Some larger materials are impacted and ground by the hammer again on the screen bar, extruded and crushed, the material is squeezed out of the gap by the hammer head of the hammer breaker to obtain the product of the required particle size. The feed inlet is equipped with a star-shaped blanking valve to control the blanking speed and prevent dust from being generated during the crushing process.
Dry Powder Cell Culture Medium Processing
The challenge for producing cell culture media lies in the huge number of recipes with lots of different components, with its different bulk density, particle size, flowability etc. A typical process steps are: Formulation » Pre-mixing » Milling » Post-mixing » Packaging.
Pudu Tech developed a cell culture medium continuous production system, with Pin Mill as the core, which integrates “Pre-mixing, Milling, Post-mixing“. And can be used to produce dry cell culture media in batches ranging from 2kg to 4000kg
·Good granularity and high uniformity
·Can be integrated with nitrogen or dry ice cooling
·Product particle size D90% is in the range of 20-150μm
·Gentle mixing, limited product heat-up
·Excellent mixing accuracy(1:100,000)
·Good cleanability, build-in nozzle (CIP)
·Central bottom outlet, complete discharge, No risk of de-mixing during discharge
Pin mill is a fine impact mill, with one or two secondary rotating disks driven by a high-speed explosion-proof motor. The disks are covered with nail-like pins. The relative speed of the outer edge pins on the rotating disk can reach 250 m/s. The pin mill achieves ultra-fine pulverization by impacting the material without destroying the crystal form of the material. The rotating disc grinding component of the pin mill is available in a variety of forms. At the same time, the fineness can be adjusted by changing the speed of the dial, so it can flexibly handle various raw materials and product fineness requirements.
In the production of cell culture media, pin mill is often used as a pulverizing component to produce ultra-fine particle size and uniform powder products. The pulverized particle size control range is 10um-200um, and the particle size range is adjustable.
·Ultra-fine milling, capable of grinding up to D97 = 35 μm
·Multi disk available, for various size range & application
·Lab & production models available
·Fabricated stainless steel, compact design & dust free operation
·Variable speed rotor drive, Energy efficient & economical to operate
·Cantilevered design for easy cleaning & rotor changes
·Maintenance free, sealed for life bearings
·Special designed seals & gas purging between drive & process area
cThe cell culture medium process system is generally divided into pre-mixing and post-mixing. According to the viscosity of the material, a cone mixer or a paddle mixer can be used.
The cone mixer is a convection mixer. Its gentle mixing force can ensure high mixing degree without destroying the material particles.
The cantilever mixing screw is connected to a cantilever, the cantilever rotates around the conical cavity, and the screw itself rotates at the same time to form convective mixing and shearing of the material. The average rotation speed of the screw itself is 70 rpm, while the rotation speed of the cantilever is 1 to 2 rpm. The linear speed of the screw is adjustable from 0.5 to 2m/s, so it is also suitable for all ATEX explosion-proof areas.
The rotating screw lifts the material from the bottom of the cavity to the top of the material. When the product particles circulate back under the action of gravity, the flow velocity of the particles will increase due to the structure of the conical cavity. The superposition of these effects provides a deep mixing and ensures a high degree of mixing performance even with low energy consumption.
In order to avoid cross-contamination between batches, the mixer is equipped with an arc or hemispherical upper cover (welded on the cavity and integrated with it), gas sealing and special internal polishing to meet cleanliness requirements.